Animal study (mouse) + cell studies · PMID 25738459

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance — VialBase Research

Discovery paper for MOTS-c as a mitochondrial-derived peptide

Last updated · 2015 · Lee C, Zeng J, Drew BG, Sallam T, Martin-Montalvo A, Wan J, Kim SJ, Mehta H, Hevener AL, de Cabo R, Cohen P · Cell Metabolism
Key findings
  • Discovery paper for MOTS-c as a mitochondrial-derived peptide
  • MOTS-c encoded within the mitochondrial 12S rRNA gene
  • Activates AMPK via inhibition of the folate cycle (AICAR accumulation)
  • Prevented age-dependent and high-fat-diet-induced insulin resistance in mice
  • Improved glucose tolerance and reduced body weight
  • Establishes mitochondria as active signaling organelles

Summary

Landmark discovery paper identifying MOTS-c as a novel mitochondrial-derived peptide encoded within the mitochondrial genome (12S rRNA). Demonstrates that MOTS-c regulates metabolic homeostasis by targeting the folate-methionine cycle, leading to AMPK activation. Exogenous MOTS-c administration prevented both age-dependent and high-fat-diet-induced insulin resistance in mice, and improved glucose tolerance.

Key Findings

  • MOTS-c is a 16-amino-acid peptide encoded by the mitochondrial genome
  • Acts as a retrograde signal from mitochondria to the nucleus
  • Inhibits AICAR transformylase (ATIC) in the folate cycle → AICAR accumulation → AMPK activation
  • Prevents age-dependent insulin resistance when administered exogenously
  • Prevents high-fat-diet-induced obesity and insulin resistance
  • Improves glucose tolerance in treated mice
  • Paradigm shift: mitochondria are not just powerhouses but active signaling organelles

Relevance to MOTS-c

This is the foundational paper for MOTS-c — all subsequent research builds on this discovery. Establishes the core mechanism (folate cycle → AICAR → AMPK), the metabolic phenotype (insulin sensitization, anti-obesity), and the conceptual framework (mitochondrial-derived peptides as retrograde signals). Essential reference for understanding MOTS-c’s biological rationale.

Citation

Lee C, et al. Cell Metab. 2015;21(3):443-454. PMID: 25738459

See Also